Expression of insulin-like growth factor-1 mRNA in rat bladder of diabetic cystopathy

Abstract

Objective To observe the insulin-like growth factor-1 (IGF-1) mRNA expression in diabetic rat bladder.Methods Thirty Sprague-Dawley (SD) male rats were randomly divided into 3 groups:normal control group,diabetic group,and treatment group,n =10 each.The rats in diabetic group and treatment group were injected intraperitoneally with 60 mg/kg Stretopzin (STZ).After 72 h,fasting blood glucose was measure,and BG ＞ 16.7 mmol/L indicated the successful establishment of diabetic model.SD rats in treatment group were given subcutaneous injection of protamine zinc insulin,so BG remained at 3.9-10.0 mmol/L.Bladders of three groups were obtained after 8 weeks,The mRNA from bladders was extracted for detection of △Ct of IGF-1 mRNA by real-time quantitative polymerase chain reaction (Real-time PCR).Results IGF-1 mRNA △Ct in the diabetic group,normal control group and treatment group was 6.141 ±0.916,4.018 ±0.491 and 4.922 ±0.788.the lower the Delta Ct value was lower,the greater the actual copy number was and the higher the amount of gene expression.Conclusion IGF-1 mRNA expression in diabetic rat bladder was significantly reduced.The insulin treatment may up-regulate the IGF-1 mRNA expression in the bladders of diabetic rats. Key words: Diabetes mellitus; Insulin-like growth factor-1 ; Bladder