Expression of indoleamine 2,3 dioxygenase enzyme in patients with sepsis during the immune inflammation progression and clinical signficance

Abstract

Objective To investigate the expression of indoleamine 2,3-dioxygenase (IDO) in patients with sepsis during the immune inflammation progression,and the role of IDO in immune inflammation in sepsis.Methods A total of 90 cases of sepsis were collected from June 2011 to October 2013 during their stay in our hospital,and divided into three groups according to their disease severity:sepsis group (n =32),severe sepsis group (n =30) and septic shock group (n =28).Thirty healthy individuals served as control group.The percentages of CD4 +,CD8 +,and CD4 + CD25 + regulatory T cells (Tregs)were detected by flow cytometry.The CD4+/CD8 + ratio was calculated.Interleukin (IL)-6,IL-10,INF-γ,and IDO concentrations were detected by enzyme-linked immunosorbent assay (ELISA).Results With the increased inflammatory response,IL-6,IL-10,INF-γ,and IDO concentrations were gradually increased,and the percentate of CD8 + Tregs was gradually increased with the difference being statistically significant among all groups (P ＜ 0.01 for all).IL-6 level was decreased,and concentrations of IL-10 and CD4 + and the CD4 +/CD8 + ratio were also gradually reduced with the difference being statistically significant among all groups (P ＜ 0.01 for all).Conclusion IDO may play an important role in immune inflammatory response,which may help to assess the severity and prognosis of sepsis. Key words: Sepsis; Immune inflammatory; Indoleamine 2,3-dioxygenase