Expression of IL-1 β and TNF-α in edematous cerebral tissues of rats after traumatic brain injury and the effect of hyperbaric oxygen on its expression

Abstract

Objective To study the changes of expression of IL-1β and TNF-αt in edematous cerebral tissues of rats after traumatic brain injury and the effect of hyperbaric oxygen(HBO)on the expression.Methods The traumatic brain injury model was established by use of graded lateral fluid percussion in rats.Minty-six rats were randomly divided into 4 groups:control group(group A),traumatic brain injury group(group B),traumatic brain injury and conventional therapy group(group C),traumatic brain injury and conventional therapy and HBO therapy group(group D)(n =24).And group D were sub divided into 4 groups:group D-3 h,group D-12 h,group D-24 h and groups D-72 h that HBO therapy was administered at 3,12,24,72 h after traumatic brain injury(n =6).Cerebral IL-1β and TNF-α protein expression and mRNA expression were tested by immunohistochemistry and RT-PCR in each group after therapy.Results The results of the cerebral protein expression and mRNA expression of IL-β and TNF-α in rats in each group,group A ＜ group D-3 h ＜ group D-12 h ＜ group D-24 h ＜ group D-72 h ＜ group C ＜ group B (all P ＜ 0.05).Conclusions IL-1β and TNF-α have a relevant relationship with the formation of brain edema after traumatic brain injury.HBO treatment can effectively improve the cognitive dysfunction through reducing the expression of IL-1β and TNF-αt after traumatic brain injury especially within 12 h after traumatic brain injury. Key words: Cerebral trauma; Hyperbaric oxygen; Interleukin-1β; Tumor necrosis factor-α