Abstract
To investigate the production of human papillomavirus type 16 (HPV16) L1 structural protein in Pichia pastoris , the effect of different gene constructs and varying cultivation conditions on production levels were assessed. The highest volumetric production levels in P. pastoris were obtained with a human codon-optimised gene construct. This construct displayed both the lowest codon adaptation index (CAI) and the highest guanine-cytosine (GC) content of the three constructs investigated. Dissolved oxygen (DO) controlled cultivations produced superior results compared to a constant methanol feeding (CMF) strategy, delivering the highest volumetric HPV L1 protein concentration (104.8 µg.l -1 ) at a 20% DO set-point. This was concomitant with an increased biomass concentration in the DO controlled cultivations.
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