Expression of HuD protein and P2Y2 receptor in the rectum of children with middle-high anorectal malformation

Abstract

Objective To observe the expression of HuD protein and P2Y2 receptor in the normal colon and the terminal rectum of children who suffered from anorectic malformation (ARM) in the middle and upper rank, and to explore the effects of HuD protein and P2Y2 receptor on the development of the enteric nervous system. Methods The terminal rectum specimens were collected from 25 children with ARM (malformation group) treated by surgerical procedures in affiliated hospital of Zunyi Medical college. In the control group, there were 15 cases. By hematoxylin and eosin (HE) staining, immunohistochemistry (IHC), immunofluorescence and Western blotting, the expression of the two proteins was detected. Results Immunohistochemistry and immunofluorescence showed that the HuD protein and P2Y2 receptor mainly existed in intestinal myenteric plexus and submucosal plexus. The integral absorbance (IA) values of HuD protein and P2Y2 receptor in control group and malformation groups were: 43.48±7.11, 3.70±1.21 and 18.83±3.65, 8.49±2.68; IA values of HuD protein and P2Y2 receptor in the two groups were: 96.56±17.38, 22.32±4.36 and 78.13±7.86, 26.56±5.32; relative quantification of HuD protein and P2Y2 receptor in two groups were 1.13±0.32, 0.49±0.12 and 0.92±0.18, 0.36±0.09 (P=0.030, 0.030). Conclusion The expression of HuD protein and P2Y2 receptor in children with ARM in the middle and upper rank was significantly lower than that in normal colon. HuD protein and P2Y2 receptor have some influence on the development of enteric nervous system in children with ARM. Key words: Anorectic malformation; Enteric nervous system; HuD protein; P2Y2 receptor