Expression of HPV16/18, p16, CK17, Ki-67 in cervical squamous epithelial lesions

Abstract

Objective To investigate the human papillomavirus 16/18 (HPV16/18) infective status and its relationship with the expression of p16, CK17, Ki-67 in immature squamous metaplasia(IM), cervical intraepithelial neoplasia (CIN) and cervical carcinomas (CC). Methods In situ hybridization (ISH) and immunohistochemistry were applied to detect the expression of HPV16/18, p16, CK17, Ki-67 on tissues from 30 IM, 60 CIN and 30 CC. Results The expression rates of HPV16/18 in LSIL, HSIL and CC were 30 % (9/30), 60 % (18/30) and 77 % (23/30), higher than that in IM [6.7 % (2/30)]. HPV16/18 was positively expressed with the progression of cervical squamous epithelial lesions. The expression rates of CK17 in IM was 90 % (27/30), higher than that in LSIL, HSIL and CC [17 % (5/30), 10 % (3/30), 6.7 % (2/30)]. The expression rates of p16, Ki-67 were 83 % (25/30) and 87 % (26/30) in LSIL, 90 % (27/30) and 93 % (29/30) in HSIL, 97 % (29/30) and 97 % (29/30) in CC, higher than that in IM [13 % (4/30) and 10 % (3/30)]. The expression of p16, Ki-67 were particularly seen in HPV16/18-positive HSIL and CC, and the correlation were observed. Conclusion HPV16/18 infection is highly associated with the degree of squamous intraepithelial neoplasia and carcinomas of cervix and upregulated p16 and Ki-67, which suggested that HPV16/18 maybe cause mutation of p16 gene. With the progression of cervical squamous epithelial lesions, positive expression rates of CK17 were decreased compared with increased of HPV16/18, p16, Ki-67. Combined detection of HPV16/18, p16, Ki-67 is helpful for diagnosis and classification of cervical squamous epithelial lesions. Key words: Human papillomavirus 16/18; CK17; p16; Ki-67; Immature squamous metaplasia; Cervical intraepithelial neoplasia; Cervical squamous cell carcinomas