Abstract
Lineages of CHO DUKX B11 cells pre-adapted to growth in serum-free suspension culture [termed PA-DUKX] have been developed and used as hosts in DNA transfections. The phenotypes of recombinant PA-DUKX lineages were qualitatively and quantitatively similar to lineages constructed using non-adapted CHO DUKX B11 cells. However, PA-DUKX cells were found to be more amenable to growth in serum-free suspension culture after transfection than non-adapted CHO DUKX B11 cells. Accordingly, the PA-DUKX host cell technology may provide an opportunity for reducing the time and resources required to develop large-scale CHO-based manufacturing processes.
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