Expression of G-protein coupled receptor 120 mRNA and related factors in subcutaneous and omental adipose tissues of obese subjects

Abstract

Objective To examine the expression of G-protein coupled receptor 120 (GPR120) mRNA in abdominal subcutaneous and omental adipose tissues and related factors in obese subjects. Methods From September 2010 to August 2011, 39 non overweight subjects (body mass index(BMI) 25 kg/m2 ) who underwent cholecystectomy in the Third Affiliated Hospital of Guangzhou Medical College were enrolled in this study. Body weight, waist-hip ratio (WHR), blood glucose, and lipids, etc, were measured. The expression of GPR120 mRNA in abdominal subcutaneous and omental adipose tissues were measured by RT-PCR. The t test, Pearson correlation analysis, partial correlation analysis and multi-stepwise regression were used for data analysis. Results (1) In obese and control group, the GPR120 mRNA expression in omental adipose tissue (0.876±0.076, 0.621±0.052) were significantly higher than those in subcutaneous adipose tissue (0.479±0.028, 0.421±0.035, t =-2.546 or -2.211, respectively, both P 0.05). (2) Multi-stepwise regression analysis demonstrated that the expression of GPR120 mRNA in omental adipose tissue was significantly correlated with HOMA-IR and WHR (R2=0.3730, standard partial regression coefficient were 0.227 and 0.321, respectively). The expression of GPR120 mRNA in abdominal subcutaneous adipose tissue was not correlated with other factors. Conclusion The GPR120 mRNA expression in abdominal omental adipose tissue was significantly higher in obese subjects than that in control group. Abdominal obesity and HOMA-IR were the main independent factors influencing the expression of GPR120 mRNA in omental adipose tissue. Key words: G-protin coupled receptor 120; Insulin resistance; Obesity; Adipose tissue