Abstract
BackgroundEmbryonic mortality during the period of implantation strongly affects litter size in pigs. Progesterone receptor (PGR) paracrine signaling has been recognized to play a significant role in embryonic implantation. IHH, NR2F2, BMP2, FKBP4 and HAND2 were proved to involve in PGR paracrine signaling. The objective of this study was to evaluate the expression of IHH, NR2F2, BMP2, FKBP4 and HAND2 in endometrium of pregnant sows and to further investigate these genes’ effect on litter size in pigs. Real-time PCR, western blot and immunostaining were used to study target genes/proteins expression in endometrium in pigs. RFLP-PCR was used to detect single nucleotide polymorphisms (SNPs) of target genes.ResultsThe results showed that the mRNA and protein expression levels of IHH, NR2F2 and BMP2 were up-regulated during implantation period (P < 0.05 or P < 0.01). All target proteins were mainly observed in luminal epithelium and glandular epithelium. Interestingly, the staining of NR2F2 and HAND2 was also strong in stroma. SNPs detection revealed that there was a -204C > A mutation in promoter region of NR2F2 gene. Three genotypes were found in Large White, Landrace and Duroc sows. A total of 1847 litter records from 625 sows genotyped at NR2F2 gene were used to analyze the total number born (TNB) and number born alive (NBA). The study of the effect on litter size suggested that sows with genotype CC tend to have higher litter size.ConclusionsThese results showed the expression patterns of genes/proteins involved in PGR paracrine signaling over implantation time. And the candidate gene for litter size was identified from genes involved in this signaling. This study could be a resource for further studies to identify the roles of these genes for embryonic implantation in pigs.Electronic supplementary materialThe online version of this article (doi:10.1186/s40104-016-0090-z) contains supplementary material, which is available to authorized users.
Highlights
Embryonic mortality during the period of implantation strongly affects litter size in pigs
A fundamental paradox of early pregnancy is that cessation of expression of Progesterone receptor (PGR) and ESR1 by uterine epithelia is a prerequisite for uterine receptivity to implantation, expression of genes by uterine epithelia and selective transport of molecules into the uterine lumen that support conceptus development
In current research, the expression patterns of genes/ proteins involved in PGR paracrine signaling over implantation time were studied
Summary
Embryonic mortality during the period of implantation strongly affects litter size in pigs. Progesterone receptor (PGR) paracrine signaling has been recognized to play a significant role in embryonic implantation. IHH, NR2F2, BMP2, FKBP4 and HAND2 were proved to involve in PGR paracrine signaling. The objective of this study was to evaluate the expression of IHH, NR2F2, BMP2, FKBP4 and HAND2 in endometrium of pregnant sows and to further investigate these genes’ effect on litter size in pigs. Implantation process is one of the important factors that affect litter size in pigs, owing to the high embryonic mortality during this stage. Indian hedgehog (IHH), which was identified as an acute PGR target gene [10], is a known member of the hedgehog (HH) signaling pathway.
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