Abstract
A new technique for coupling poly(Glu 60 Ala 30 Tyr 10) (GAT) to sheep red blood cells (SRBC) is reported; p-benzoquinone has been used as a coupling agent. The anti-GAT immune response of BALB/c mice has been investigated at the PFC level using these indicator cells (GAT-BQ-SRBC). The kinetics of the primary and secondary in vivo anti-GAT immune response has been investigated: IgM and IgG plaque-forming cells are detectable as well as IgM nonhemolytic PFC's. With respect to the anti-GAT immune response in vitro, only a secondary response can be detectable on GAT-BQ-SRBC. These results contrast with previously reported observations concerning the anti-GAT response measured on GAT-coupled SRBC using the chromium chloride technique. All the GAT-specific PFC from BALB/c mice are inhibitable by anti-idiotypic serum 715-10A prepared against anti-GAT antibodies. The specificity of this inhibition has been verified. Finally we have determined that all the antiGAT PFC from various strains of mice with different allotypic markers can also be inhibited by 715-10A serum. These results confirm the broad distribution of GAT-715 idiotype and establish that the entire anti-GAT repertoire of the mouse species is limited to antibodies with idiotypic determinants cross-reacting with GAT-715.
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