Abstract
In the present study, we provide evidence for the expression of all three GABA C receptor ρ subunits through development of the rat cerebellum. Injection of cerebellum mRNA into frog oocytes gave rise to the expression of both GABA A and GABA C receptors. qRT-PCR of RNA isolated from postnatal developing cerebella showed that the expression of each ρ subunit is relatively low, with a relative comparative expression of ρ3 > ρ1 > ρ2. In situ hybridization and immunohistochemistry revealed a limited distribution of GABA C receptors in the Purkinje and Golgi neurons whereas electron microscopy detected the ρ1 and ρ2 subunits in the soma and dendritic tree of the Purkinje cells. The expression of GABA C receptors in the cerebellum adds a new dimension to the regulation of GABAergic neurotransmission and suggests further experiments to determine their functional consequences.
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