Expression of FOXA1 and GATA3 correlates with invasivity and pathological stage a grade of primary transitional cell carcinoma of the bladder

Abstract

Bladder cancer is the fifth most common malignancy in men and the second most commonly diagnosed urogenital tumor. Approximately 400,000 new patients with this disease occur each year in the world. Incidence rates are 4 times higher in men than in women. Diagnosis is based on cystoskopy, radiological examination and histological examination. Histological evaluation provides definitive diagnosis and also provides information about pathological stage and grade. Recently, methods of molecular biology are used to predict biological behavior an prognosis more precisely. In this study we evaluated 73 patients with primary transitional cell carcinoma (TCC) of urinary bladder (39 patients pTa and 34 pT1 TCC). Total RNA was extracted from bladder tissue samples using GeneJET RNA Purification Kit (Thermo Scientific, USA) according to the manufacturer's recommendations. First‐strand cDNA was synthesized from total RNA with Maxima First Strand cDNA Synthesis Kit for RT‐qPCR (Thermo Scientific, USA). Reaction was performed according to protocol recommended by manufacturer. Obtained cDNA was used as a template for quantitative PCR to determine the expression level of selected genes (TP53, BCL2, FOXA1 and GATA3). The PCR reactions were performed on Eco Real‐Time PCR System (Illumina, USA). Expression of all analyzed genes was normalized to housekeeping gene, glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH). Obtained results showed that expression levels of TP53 and BCL2 showed no significant differences in both groups. Conversely, significantly lower expression of FOXA1 and GATA3 were detected in pT1 TCC when compared to pTa TCC. Moreover, significantly higher expression of FOXA1 and GATA3 was detected in high grade TCC when compared to low grade tumors. Based on our findings, expression of FOXA1 and GATA3 correlates with invasivity and pathological stage a grade of primary bladder TCC, and may be used for further research in prospective studies.Support or Funding InformationVEGA No. 1/0207/16.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.