Expression of EZH2 gene in cervical squamous carcinoma and its clinicopathologic significance

Abstract

Objective To study the expression of EZH2 gene in cervical squamous carcinoma and its clinicopathologic significance. Methods The expression of EZH2 mRNA was detected in 21 samples of normal cervical tissue, 27 samples of CIN tissue and 48 samples of cervical squamous carcinoma tissue by RT-PCR. And the relationship between EZH2 expression and the clinical pathological characteristic was analyzed. Results The level of EZH2 mRNA in cervical squamous carcinoma tissues (1.67±0.01) were significantly higher than that in the normal cervical tissues (0) and CIN tissues (0.36±0.02) (P ＜ 0.01).There was no correlation between EZH2 and ages (P ＞ 0.05), while the expression of EZH2 was highly correlated with histologic stage,clinical stage,muscular invasion depth and lymph node metastasis of cervical squamous carcinoma (P ＜ 0.01).Conclusion The over-expression of the EZH2mRNA may play an important role in the pathogenesis and progression of cervical squamous carcinoma, suggesting that EZH2 might be a new biomarker for diagnosing cervical squamous carcinoma. Key words: Gene,EZH2; Cervix uteri; Carcinoma,squamous cell; Eeverse transcriptase polymerase chain reaction