Abstract
Abstract Papain treatment of Epstein-Barr virus (EBV; (P3HR-1 strain)-superinfected Raji cells removed the initially adsorbed membrane antigen (MA) positive material from the cell membranes. MA positive cells appeared again after 20 hr in culture, reaching a maximum level at about 30 hr. Puromycin, cycloheximide, and actinomycin D prevented the appearance of MA, whereas cytosine arabinoside had no effect. These results suggest that MA is synthesized de novo by P3HR-1 virus-infected cells and can be detected after 20 hr. The effects of the different metabolic inhibitors are in line with the concept that MA synthesis is an early function of the viral genome. Parallel EA induction tests showed that papain treatment had no effect on the frequency of EA positive cells compared to buffer-treated cells. A comparison between P3HR-1 and B95-8 virus strains with equal EBNA-inducing capacity showed that B95-8 virus was deficient with regard to its ability to induce MA in Raji cells, as judged by direct immunofluorescence.
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