Expression of epidermal growth factor receptor and kirsten rat sarcoma viral oncogene in non-small cell lung cancer lymph node metastasis and the clinical significance

Abstract

Objective To investigate epidermal growth factor receptor(EGFR)and kirsten rat sarcoma viral oncogene(KRAS)expression in non-small cell lung cancer(NSCLC) and lymphatic metastases, and to explore the roles of EGFR and KRAS in NSCLC lymphatic metastases. Methods By collecting surgically resected and pathologically confirmed cases of NSCLC, get 186 cases of complete clinical data,extraction of tumor cell DNA, polymerase chain reaction(PCR)amplification and sequencing.To observe EGFR and KRASexpression in NSCLCand lymphatic metastasis,and to analyse with SPSS 14. 0 statistical software. Results Positive rate of EGFR gene expression in NSCLC was 34. 41%(64 cases).EGFR gene expression was positively correlated with the differentiation and lymphaden metastases.Positive rate of KRAS expression in gastric cancer was 36. 56%(68 cases).KRAS expression was positively correlated with the lymphaden metastases and clinical stages.The mutation rate of KRAS had significant difference in NSCLC and lymph node metastasis, but the mutation rate of EGFR had no significant difference. Conclusion EGFR and KRAS were high expression in NSCLC tissue, and it may give some guideline for the clinical diagnosis,illness evaluation, treatment and prognosis of gastric cancer. Key words: Non-small cell lung cancer; Epidermal growth factor receptor; Kirsten rat sarcoma viral oncogene; Lymphatic metastasis