Abstract
PurposeThe present study compared the expression of early growth responsive gene-1 (Egr-1) in visual cortex between amblyopia kittens and normal kittens, and to explore the role of Egr-1 in the pathogenesis of amblyopia.MethodsA total of 20 healthy kittens were randomly divided into deprivation group and control group with 10 kittens in each group. Raised in natural light, and covered the right eye of the deprived kittens with a black opaque covering cloth. Pattern visual evoked potentials (PVEP) were measured before and at the 1st, 3rd and 5th week after covering in all kittens. After the last PVEP test, all kittens were killed. The expression of Egr-1 in the visual cortex of the two groups was compared by immunohistochemistry and in situ hybridization.ResultsPVEP detection showed that at the age of 6 and 8 weeks, the P100 wave latency in the right eye of deprivation group was higher than that in the left eye of deprivation group (P < 0.05) and the right eye of control group (P < 0.05), while the amplitude decreased (P < 0.05). The number of positive cells (P < 0.05) and mean optical density (P < 0.05) of Egr-1 protein expression in visual cortex of 8-week-old deprivation group were lower than those of normal group, as well as the number (P < 0.05) and mean optical density of Egr-1 mRNA-positive cells (P < 0.05).ConclusionsMonocular form deprivation amblyopia can lead to the decrease of Egr-1 protein and mRNA expression in visual cortex, and then promote the occurrence and development of amblyopia.
Highlights
Amblyopia is one of the important diseases causing visual loss of children in the world, and its incidence rate in Asia is 1.09% [1]
P100 wave of Pattern visual evoked potentials (PVEP) The PVEP examination showed that the main wave image of kitten PVEP showed N75-P100-N135 complex wave, which was composed of two positive waves and one negative wave, showing an “M” shape (Fig. 2)
At the age of 3 weeks, there was no statistical difference in latency (F = 0.254, P = 0.778) and amplitude (F = 0.009, P = 0.990) of P100 wave between right eye and left eye of deprivation group and right eye of control group
Summary
Amblyopia is one of the important diseases causing visual loss of children in the world, and its incidence rate in Asia is 1.09% [1]. The plasticity mechanism of visual development is related to Synapse is currently considered as the most critical link in amblyopia, and its plasticity can be divided into long-term potentiation (LTP) and long-term depression (LTD) according to time [7, 8]. The increase of Egr-1 expression is associated with synaptic plasticity, memory consolidation, LTP induction and learning [9, 10]. Immediate early genes have the effect of coupling short-term signals with long-term changes
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