Abstract
To discuss the significance of DOG1, CD117 and PDGFRA in the diagnosis of gastrointestinal stromal tumors (GISTs), and analyze their correlations with clinicopathological features and risk ranking. DOG1, CD117 and PDGFRA were detected with IHC Envision ldpe-g-nvp in 63 GISTs and 43 cases of non-GISTs, and analyzed for relations with clinicopathological factors (gender, age, location, tumor size, mitotic phase, histology) and risk degree. The positive expression rate of DOG1, CD117 and PDGFRA in GISTs was 84.1% (53/63), 90.5% (57/63), 53.2% (33/63), respectively. Among the 6 CD117 negative cases, all were DOG1 positive and 5 were PDGFRA positive. Rates in patients with non-GISTs was 11.6%, 16.3%, 6.98%, respectively. Expression of DOG1 and PDGFRA demonstrated no significant variation with gender, age, position, tumor size, mitotic phase, histology, and risk rank. However, CD117 was related with position and histology (P=0.008 and P=0.045), those in the mesentery having a higher positive rate than those derived from stomach, small intestine, colon and rectum (50.0% vs 94.7%, P=0.008). Furthermore CD117 was also highly expressed in spindle and epithele types. DOG1 had a good sensitivity and specificity as a kind of newly discovered marker, especially for KIT negative GISTs. However, DOG1, CD117 and PDGFRA cannot be used for assessing the rish of patients.
Highlights
Gastrointestinal stromal tumors (GISTs) are a special kind of tumors which is derived from mesenchymal tissues of gastrointestinal tract
DOG1, CD117 and PDGFRA were detected with IHC Envision ldpe-g-nvp in 63 gastrointestinal stromal tumors (GISTs) and 43 cases of non-GISTs, and analyzed for relations with clinicopathological factors and risk degree
Localization of DOG1, CD117, AND PDGFRA in tissues of GISTs patients (Table 2): In the samples of GISTs, the staining of GOD1 were mainly localized in cyroplasma, a few were localized in the cytomembrane, CD117 was mainly in the cytoplasma and a few in the cytomembrane, too, and PDGERA was mainly in the cytoplasma
Summary
Gastrointestinal stromal tumors (GISTs) are a special kind of tumors which is derived from mesenchymal tissues of gastrointestinal tract. The high expression of DOG1 in GISTs indicates its importance in the tumorigenesis and tumor developments, and DOG1 may be a potential marker for tumor diagnosis. The high sensitivity and specificity makes DOG1 an important diagnose evidence, the combined detection of GISTs, CD117, and PDGFRA can provide more accurate diagnose evidences both in pathology and in clinic. We detected the expression of DOG1 in 63 tissues of patients with GISTs and 43 tissues of patients with non-GISTs by immunohistochemistry to explore the clinical significance of the immunohistochemistry results and relative clinic pathologic factors and the correlation with the tumor stages. We suppose to improve the detection rate of GISTs and reduce the misdiagnose rate
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