Expression of DNA binding protein A in colorectal carcinoma tissues and cells and its clinical significance

Abstract

Objective To investigate the expression of DNA binding protein A (dbpA) in patients with colorectal carcinoma of different stages and its significance. Methods Expression of dbpA protein and mRNA in specimens of normal tissues and colorectal cancer were detected by immunohistochemistry, expression of dbpA mRNA and protein of colorectal cancer cell lines SW480, RKO, SW620, DLD-1, HT-29, SW1463 and tissues were detected by immunohistochemistry, RT-PCR and Western blot. Results There was no positive staining dbpA protein and mRNA in normal colorectal tissues. However, dbpA was expressed in epithelial cells of colorectal mucosa [dbpA mRNA: 80.0 % (48/60), 10.0 % (6/60); dbpA protein: 83.3 % (50/60), 10.0 % (6/60), P< 0.01]. And there was no expression in normal colorectal cell, but its expression was high in 6 colorectal cancer cells (P< 0.05). The high expression of dbpA was correlation with the infiltration depth, lymph node metastasis and type of histology (P< 0.05), and had effect in prognosis of colorectal cancer (P< 0.05). Conclusion Elevated dbpA may be related to the pathogenesis and development of colorectal carcinoma, and dbpA may be a prognostic factor of colorectal carcinoma. Key words: Colorectal neoplasms; DNA binding protein A; Immunohistochemistry; Reverse transcriptase polymerase chain reaction; Blotting, western