Abstract
Objective To investigate the expression of DMBT1 in liver cirrhosis and its relation to oval cell proliferation.Methods Immunostaining was carried out for CK19, Hep and DMBT1 in 22 liver cirrhotic samples.The homozygous deletion of DMBT1 in proliferated bile ductules (PBD) was investigated by laser capture microdissection (LCM).Meanwhile, the samples from 5 cases were ex-amined with transmission electron microscopy.Ten normal liver tissues were used as controls.Results Microscopically, oval cells could be found in all cirrhotic cases which could coexpress CK19 and Hep and its number was found to increase significantly with the progression of inflammation.Electron mi-croscope identified the presence of oval cells in PBD.DMBT1 could express in these cells too and its positive rate in PBD was 45.5 %.The homozygous deletion of DMBT1 was found in 45.5% PBD.In normal liver tissues, PBD and oval cells were not found.DMBT1 expression was only detectable in few large bile ducts.Conclusion There are bipotent oval cells with high expression of DMBT1 in liver cirrhosis hiding in PBD and DMBT1 can be specifically deleted in PBD, suggesting that DMBT1 may be related to the proliferation of oval cells and may participate in the development of liver cirrhosis. Key words: Liver cirrhosis; Oval cell; DMBT1; Laser capture microdisseetion; Allelic deletion
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