Expression of Dishevelled2 gene and protein in clear-cell renal cell carcinoma tissues and its clinicalsignificance

Abstract

：Objective To explore therelationship of DVL2 expression and the development of (CCRCC) by comparing the changes ofDVL2 mRNA and protein expression in CCRCC specimens and matched normal renal specimens andits clinical significance. Methods DVL2 mRNA expressions in 22 CCRCC tissues, the matchedadjacent normal tissues, and 10 CCRCC tissues alone were examined by semiquantitativeRT-PCR and fluorescence quantitative PCR (real-time RT-PCR). Meanwhile, the differentexpression of the CCRCC between TNM Stage Ⅲ+ Ⅳ and Stage Ⅰ +Ⅱ was also examined.Furthermore,immunohistochemistry was employed to examine DVL2 protein expression in 22CCRCC and the matched adjacent normal tissues, and the other 10 CCRCC tissuses without thematched tissues. Results The DVL2 mRNA expression levels in 17 CCRCC tissues wereincreased by semi-quantitative RT-PCR and by real time RT-PCR compared with that incorresponding adjacent normal tissues, with the difference being significantly different(t = 2.535, P =0.0197). The DVL2 expression of 8 in 13 Ⅲ + ⅣCCRCC was higher than Ⅰ+ⅡCCRCC. Immunohistochemical examination showed that theDVL2 protein was located in cytomembrane and cytoplasm. Moreover, the positive level ofDVL2 protein in CCRCC tissues[81.8 % (18/22)]was significantly higher than those in theadjacent tissues. However the expression was not associated with patients' age, gender,TNM stages (Fisher exact frenquently, P >0.05). Conclusion The DVL2 expression in CCRCCis obviously higher than the corresponding normal tissues in the level of mRNA andprotein. And the higher DVL2 expression might be closely associated with the developmentand progression of CCRCC in the level of mRNA, which may be a potential molecular markerof CCRCC development and metastasis mechanism. Key words: Clear cell renal cellcarcinoma; Immunohistochemistry; Dishevelled 2 protein