Expression of digestive enzymes and nutrient transporters in the intestine of Eimeria maxima-infected chickens

Abstract

Infection with the intestinal protozoa Eimeria causes destruction of intestinal epithelia, resulting in reduced feed efficiency and BW gain. The objective of this study was to investigate the effect of Eimeria maxima infection on the expression of 20 digestive enzymes as well as macro- and micronutrient transporters in the intestine. Expression of the brushborder membrane amino acid transporters EAAT3 (excitatory amino acid transporter 3) and bo+ AT (Na+-independent neutral amino acid transporter) was decreased to 35 to 39% and 20% of control, respectively, in the intestine of E. maxima-infected chickens. Expression of the basolateral amino acid transporter LAT1 and the amino acid transporter ASCT1 was upregulated 17- to 19-fold and 12-fold, respectively, in E. maxima-infected chickens, whereas the zinc transporter was decreased to 20 to 44% of control. In addition, expression of the antimicrobial peptide LEAP-2 (liver-expressed antimicrobial peptide-2) was reduced to 5 to 24% of control. The other 13 digestive enzymes or nutrient transporters examined were unaffected. Together these results suggest a model whereby, upon infection, Eimeria causes a downregulation of LEAP-2. In response, there are changes in expression of the amino acid transporters that would result in a depletion of the energy source (glutamate) and some essential amino acids, which may lead to death of the cell and inhibition of pathogen replication.