Expression of DEC2 enhances chemosensitivity by inhibiting STAT5A in gastric cancer.

Abstract

Gastric cancer (GC) is one of the most common cancers. Resistance to 5-fluorouracil (5-Fu)-based chemotherapy is a major cause of treatment failure followed by the poor prognosis of patients. In GC, it was reported that human differentiated embryonic chondrocyte-expressed gene 2 (DEC2), suppressed tumor proliferation and metastasis, but the effect of DEC2 on chemosensitivity of GC cells was unknown. In our study, we found that DEC2 can obviously increase the sensibility of GC cells to 5-Fu by promoting 5-Fu-induced apoptosis. DEC2 overexpression is significantly associated with decreased phosphorylation of STAT5A (P-STAT5A). More importantly, negative correlations between DEC2 with P-STAT5A expression were observed in tissue sections from GC patients. GC patients with low expression levels of DEC2 and high expression levels of P-STAT5A showed a poor prognosis. Furthermore, enhanced chemosensitivity mediated by DEC2 can be reversed by STAT5A which confer GC cells resistance to apoptosis induced by 5-Fu. Together, our results suggest that through inhibiting activation of STAT5A, DEC2 enhances 5-Fu-induced apoptosis and suppression of proliferation in GC cells. These findings will provide new insight for identifying potential targets that can be used to sensitize GC cells to chemotherapy.