Expression of cytidine triphosphate synthetase 1 in pancreatic cancer cell lines and its clinical significance

Abstract

Objective To investigate the cytidine triphosphate synthetase 1(CTPS1) expression in pancreatic cancer cell lines and its impaction on prognosis. Methods The GEO profiles database was searched to collect clinical data of 39 patients of pancreatic ductal adenocarcinoma . Kaplan-Meier curve was used to analyze the relationship between the patients′ prognosis and the expression of CTPS1 . qPCR and Western blot were applied to detect the expression of CTPS1 in 4 pancreatic cancer cell lines. Results The mRNA expression of CTPS1 in Sw1990, BxPC-3, Panc1, and MIA-Capa2 was 1.00±0.20, 2.92±0.95, 4.29±0.14, and 2.26±0.33 (t=-16.19, -11.45, -8.09, -13.12, P<0.05) when compared to the normal pancreatic ductal epithelial cell line HPDE of 7.70±0.72. The relative protein expression of CTPS1 in pancreatic cancer cell lines Sw1990, BxPC-3, Panc1, and MIA-Capa2 was 0.40±0.06, 0.20±0.09, 0.68±0.11, and 0.48±0.06 (t=-8.97, -10.5, -4.39, -7.88, P<0.05) when compared to HPDE of 1.09±0.12. The overall survival (OS) in patients with higher CTPS1 expression than adjacent tissue was 28 months (95% CI: 9.2-46.8 months), and that with lower CTPS1 expression was 13 months (95% CI: 8.2-17.8 month), (χ2=4.02, P<0.05). Conclusions CTPS1 in the pancreatic cancer cell lines are of low expression, and the level is positively related to patients′ survival time. Key words: Cytosine nucleotide; Pancreatic neoplasms; Prognosis