Expression of CXCR4 predicts lymph node metastasis in early breast cancer

Abstract

9525 Background: Recent experimental studies have suggested that interactions between the chemokine receptor CXCR4 and its ligand CXCL12 in host organs may play a role in breast cancer progression. There is currently no data if CXCR4 expression is associated with metastatic potential in early breast cancer. Therefore, we studied the differential expression of CXCR4 in small size lymph node positive and lymph node negative breast cancers to evaluate whether CXCR4 predicts metastasis to axillary lymph nodes. Methods: CXCR4 expression was evaluated on paraffin-embedded tissue sections obtained from patients with T1N0 (n=98) and T1N1 (n=99) breast cancer, using immunohistochemical staining. The intensity, staining percentage, and localization pattern (nuclear, cytoplasmic) were assessed, and positivity was categorized as follows: high cytoplasmic only versus others, and predominantly nuclear versus others. The expression patterns were correlated with lymph node status, and with other prognostic markers includ...