Abstract
This study is to explore the expression of CPLX1 (Complexin 1) in spinal cord tissues with different degrees of injury in the subacute phase of spinal cord injury. Twenty-four SD rats were randomly divided into three experimental groups (light, medium and severe injury) and a sham-operated control group. The experimental group made acute spinal cord injury models according to the lisa method, and the spinal cord tissue were collected 72 hours after injury. The tissues of rats were taken for transcriptome high-throughput sequencing and proteomic analysis experiments to observe the expression of CPLX1 in the different degrees during the subacute phase of spinal cord injury. The mRNA and protein of CPLX1 were detect in rat spinal cord tissue, and they were expressed high in the sham operation group. The protein expression in the light injury group began to decrease, and with the increase of the degree of injury, the transcription level of CPLX1 and the amount of protein expression also gradually decreased. The change trend of mRNA transcription level and protein expression level was basically the same. When the spinal cord injury heavier, the less CPLX1 was expressed, the two showed a significant negative correlation. In the subacute stage of spinal cord injury, the translation and protein levels of CPLX1 decreased significantly. The more severe the injury, the lower the transcription level and protein expression level. CPLX1 may be used as a marker gene for the severity of spinal injury.
Highlights
With the development of modern society, the incidence of spinal cord injury due to traffic accidents, high falls and sports injuries has increased year by year [1]
Transcriptome high-throughput sequencing of four differently injured spinal cord tissues in the subacute phase showed that the expression level of Cplx1 was higher in the sham operation group, and as the degree of injury deepened, the transcription level of Cplx1 became lower and lower
The results showed that the correlation between mRNA and protein expression of Complexin 1 (CPLX1) in the subacute phase of spinal cord injury conforms to the formula Y=0.001X-2.795, and the r value is 0.911, which indicated that mRNA expression and protein 'S expression showed a clear positive correlation
Summary
With the development of modern society, the incidence of spinal cord injury due to traffic accidents, high falls and sports injuries has increased year by year [1]. Spinal cord injury is an extremely serious nervous system injury [2]. There are corresponding changes in dystonia and positive pathological reflexes [3]. Various motor and sensory dysfunctions occur in the corresponding segments of the injury, and the disability rate is very high, seriously affecting the patient’s quality of life, bringing a heavy psychological and economic burden [4]. Current methods for treating spinal cord injury include drugs, physiotherapy, and surgery, but have not been able to make breakthrough progress [5,6,7]. The main reason is that the molecular mechanism of cells after spinal cord injury is still unclear
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