Expression of Connexin 43 and E-cadherin Protein and mRNA in Non-small Cell Lung Cancers in Chinese Patients

Jun-Qiang Zhao,Jia-Xiang Wang,Shan-Shan Liu,Jun Yang,Yu-Quan Wu,Fang-Jie Sun,Gui-Shan Li,Qing-Yong Chen

doi:10.7314/apjcp.2013.14.2.639

Abstract

Connexin 43 (Cx43) and E-cadherin are important biomarkers related with cancer. Their expression at protein and mRNA levels was here investigated in 50 primary lung carcinoma tissues and 20 samples of adjacent normal tissue of Chinese patients with non-small cell lung cancer (NSCLC). Protein and mRNA expression were evaluated by ABC immunohistochemistry and RT-PCR. (1) The positive expression rates of Cx43 and E-cadherin protein were higher in the adjacent normal tissues than those in the primary lung carcinoma tissues; (2) the positive expression rates of Cx43 and E-cadherin protein decreased with NSCLC progression; (3) the expression of E-cadherin protein was not related with the pathological type of NSCLC; and (4) the relative quantity of the Cx43 or E-cadherin mRNA expression was correlated with the the histological type, clinical stage, cancer cell differentiation and the lymph node metastasis. The data suggested that the Cx43 and E-cadherin are reduced with NSCLC progression, and might be important biomarkers for judging the metastasis and prognosis.

Highlights

Lung cancer is the most common cancer in the world
Their expression at protein and mRNA levels was here investigated in 50 primary lung carcinoma tissues and 20 samples of adjacent normal tissue of Chinese patients with non-small cell lung cancer (NSCLC)
Results: (1) The positive expression rates of Connexin 43 (Cx43) and E-cadherin protein were higher in the adjacent normal tissues than those in the primary lung carcinoma tissues; (2) the positive expression rates of Cx43 and E-cadherin protein decreased with NSCLC progression; (3) the expression of E-cadherin protein was not related with the pathological type of NSCLC; and (4) the relative quantity of the Cx43 or E-cadherin mRNA expression was correlated with the the histological type, clinical stage, cancer cell differentiation and the lymph node metastasis

Summary

Introduction

Lung cancer is the most common cancer in the world. Non-small cell lung cancer (NSCLC) is the most common bronchial tumor, which can be classified in two major histological subtypes, the adenocarcinoma (AC) and the squamous cell carcinoma (SCC). Lung cancer rates are increasing in China (Parkin, 2001; Jemal et al, 2011). Connexins was a group of homologous proteins which from the inter membrane channels of gap junctions (Proksch et al, 2008). The variety contributes to the wide range of functional properties of gap junction (Sohl and Willecke, 2004; Laird, 2006; Solan and Lampe, 2009)

Objectives

Methods

Results