Abstract
Background. Postoperative adhesions are a serious problem in surgery. However, at the present time molecular
 mechanisms of the adhesion process are insufficiently studied.
 Aim. To study the dynamics of expression of genes encoding the synthesis of collagen in case of damage to the serosa
 on the example of the peritoneum in conditions of aseptic inflammation.
 Materials and methods. Aseptic inflammatory process in the abdominal cavity was modeled (Wistar rats, n = 40).
 A micro- and macroscopic picture of the damage area was studied. Immunofluorescent staining for Type I collagen
 (Col 1A1) was performed. The expression of genes encoding collagen of different types was evaluated using the RT 2 -
 Profiler PCR kit Array Rat Wound Healing.
 Results. It has been established that the adhesion process with peritoneal damage in aseptic conditions reaches its
 maximum by the 30 th day of observation. The same period coincides with the maximum of collagen synthesis in fibro-
 blasts in the repair area, revealed by immunofluorescence study. The interrelation of synthesis of type I and III collagens
 went as expected – the onset of expression of type III collagen (from day 3) is ahead of the expression of collagen type I
 (from day 7). Peak gene expression of collagens type I, Alpha-1 and -2; type III Alpha-1, type IV Alpha-1 and -3, type V
 Alpha-1, -2 and -3; type XIV Alpha-1 (Col14a1) falls on the 14th day. For the first time, active involvement of type V
 alpha-3 collagen in the adhesion process was noted - we detected both early (from day 1) and maximum intensive (up
 to 166.96 times increase in comparison with intact animals).
 Conclusion. Perhaps, the hyperexpression of collagen V alpha-3 that we revealed is an important link in the pathogenesis
 of adhesion in the abdominal cavity.
Highlights
Postoperative adhesions are a serious problem in surgery
The same period coincides with the maximum of collagen synthesis in fibroblasts in the repair area, as revealed by immunofluorescence study
A decrease in the expression of collagen genes practically to “background” values in the injury area indicates the lack of potential for further growth of collagen synthesis and the completion of the adhesion process in the abdominal cavity
Summary
Postoperative adhesions are a serious problem in surgery. The causes of adhesive process could be trauma, bacterial infection, bleeding, and foreign bodies in the abdominal cavity. 28 types of collagen are distinguished, which are encoded by more than 40 genes. They differ in molecular organization, organ and tissue affiliation. Aim. To study the dynamics of expression of genes encoding the synthesis of collagen in case of damage to the serosa on the example of the peritoneum in conditions of aseptic inflammation. It has been established that the adhesion process with peritoneal damage in aseptic conditions reaches its maximum by the 30th day of observation. Active involvement of type V alpha-3 collagen in the adhesion process was noted - we detected both early (from day 1) and maximum intensive (up to 166.96 times increase in comparison with intact animals). The hyperexpression of collagen V alpha-3 that we revealed is an important link in the pathogenesis of adhesion in the abdominal cavity
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
