Expression of collagen type I in scleral extracelluar matrix tissue at the anterior and posterior poles in the normal Chinese eye

Abstract

Objective To analyze whether or not there is a difference in the expression of collagen type I scleral extracellular matrix tissue at the anterior and posterior poles and to investigate the influence of seleral tissue in resisting the force of elongation in order to explore objective reasons for pathological myopia pathogenesis. Methods Trabeculectomy was used to obtain scleral tissue samples at the anterior and posterior poles of 15 healthy volunteers （without pathological myopia） who had donated their corneas. The expression of collagen type I was detected by Western blot, recording the IOD of β-actin and collagen type I in accordance with the electrophoresis strips＇ gray scale values of β-actin and collagen type I and the ratios of the two （collagen type I/β-actin）, Results The protein band of collagen type I was 100 KD. There was a significant difference between the two groups （P〈0.01）, Conclusion There was a significant difference in scleral tissue at the posterior and anterior poles in the normal eye. The expression of collagen type I was significantly lower at the posterior pole compared to the anterior pole. Key words: scleral tissue; pathological myopia; collagen type I ; Western blot