Abstract
目的 研究肛门直肠畸形(ARM)患儿直肠末端c-kit和SCF的表达水平,以探讨ARM患儿术后便秘的分子机制.方法 通过RT-PCR方法研究10例高位、10例中位、8例低位ARM畸形和2例后天性肛瘘直肠末端c-kit mRNA和SCF mRNA的表达水平,并与正常对照组进行比较.结果 与正常对照组相比较,c-kit mRNA、SCF mRNA的表达在高位ARM组、中位ARM组差异有显著意义(P<0.05),后天性肛瘘组、低位ARM组差异无显著意义(P>0.05).结论 低位ARM和后天性肛瘘直肠末端c-kit mRNA和SCF mRNA表达未见明显异常提示这两种疾病可能不影响患儿直肠末端Cajal间质细胞的发育和功能.高、中位ARM患儿直肠末端c-kit mRNA和SCF mRNA表达的减少可能是先天性的,将影响Cajal间质细胞的发育和功能,可能是患儿术后便秘产生的分子生物学证据之一。
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