Expression of centromere protein-C (CENP-C) in villus tissue of the first-trimester spontaneous abortion and its correlation with chromosome segregation

Abstract

Objective To investigate the expression of centromere protein-C (CENP-C) in villus tissue of the first-trimester spontaneous abortion (SA) and the correlation study of CENP-C expression with chromosome segregation. Methods Fluorescence in situ hybridization (FISH) and G-banded karyotype analysis were used to detect the numerical chromosome abnormality in 94 villus tissues of women with SA. The participants were separated into case group (n=30) and control group (n=30) according to the results with FISH. The qRT-PCR and Western blotting analysis were used to assess the expression level of CENP-C. Results Forty-eight (51.06%) cases had observed the numerical chromosome abnormality, including 30 positive cases and the positive rate was 31.91%. The main types of variation included trisomy 16, 21, 22, X monosomy and triploid. The expression levels of CENP-C mRNA and protein in case group were statistically higher than that in control group (P Conclusion Expression of CENP-C in the villus tissues of women might be related to SA induced by chromosomal aneuploid.