Expression of CD98 in colorectal cancer and its relationship with CD147

Abstract

Objective To investigate the protein exp peopletracellular metalloproteinase inducer (CD147) in human colorectal cancer tissues. Methods EnVision immunohistochemical method was used to detect the expression of CD98 protein in 70 cases of colorectal cancer and their adjacent tissues. Western blotting was used to detect the expression of CD98 and CD147 protein in HCT116 cells after transfection with CD98 small interfering RNA (siRNA) and negative control. Results The positive rate of CD98 in colorectal cancer and the adjacent tissues was 57.1% (40/70) and 40.0% (28/70), which was statistically significant (P=0.042). The expression of CD98 in colorectal cancer was closely correlated with tumor stage (P=0.017) and lymph node metastasis (P=0.017), but not with gender (P=0.125), age (P=0.935), distant metastasis (P=0.464), tumor differentiation (P=0.995). There was a significant positive correlation between the expression of CD98 and CD147 in colorectal cancer (r=0.471, P=0.001). The expression of CD98 and CD147 protein was obviously decreased in HCT116 cells after transfection with CD98 siRNA, compared with negative control. Conclusion The expression of CD98 is closely related to the invasion and metastasis of colorectal cancer. CD98 may regulate the invasion and metastasis of colorectal cancer by up-regulating the expression of CD147. Key words: Colorectal cancer; CD147; CD98; Immunohistochemistry; Cell transfection