Expression of CD63 and melanoma antigen-1 and its clinicopathological significance in benign and malignant lesions of gallbladder

Abstract

Objective To study the expression of CD63 and melanoma antigen-1(MAGE-1) and explore their clinicopathological significance in the benign and malignatnt lesions of gallbladder. Methods EnVisiom immunohistochemical method was used for detecting the expression levels of CD63 and MAGE-1 in the routinely paraffin-embedded sections of the resected specimens with gallbladder adenocarcinoma(n=108), peritumoral tissues(n = 46), adenomatous polyp(n = 15) and chronic cholecystitis(n=35). Results The positive rate of CD63 was significantly lower in gallbladder adenocarcinoma than in peritumoral tissues(X~2=15.77, P(0.01), adenomatous polyp(X~2=10. 81,P<0.01)and chronic cholecystitis(X~2= 26.47, P<0.01). The positire rate of MAGE-1 was significantly higher in gallbladder adenocarcinoma than in peritumoral tissues(X~2=31.27, P<0. 01),adenomatous polyp(X~2= 13.57, P<0. 01), and chronic cholecystistis(X~2=35.47, P<0. 01). Thepositive rate of CD63 was significantly higher in the adenomatous canceration, the maximal diameter of mass(2 cm, no-metastasis of lymphnode, no-infiltration of reginal tissues or organs than those in the moderately-or low-differentiated adenocarcinoma, the maximal diameter of mass≥ 2 cm, metastasis of lymphnode, and infiltration of regional tissues or organ(P<0.05 or P<0. 01). But the expression of MAGE1 was the opposite compared with CD63 in gallbladder adenocarcinoma(P<0. 05 or P<0. 01). Conclusion The expression levels of CD63 and/or MAGE-1 is closely related to the carcinogenesis, progression, metastasis, infiltrating potential and prognosis of gallbladder adenocarcinoma. Key words: Gallbladder neoplasm; Gallbladder polyp; Chronic cholecystitis; CD63; Melanoma antigen-1; Immunohistochemistry