Abstract
Objective:To explore the relationships between expression of CD44v6, lymphatic vessel density (LVD) and the clinicopathological parameters of patients.Methods:One hundred early gastric cancer tissues, 55 high-grade gastric intraepithelial neoplasia (HGIN) tissues, 60 low-grade gastric intraepithelial neoplasia (LGIN) tissues and 60 chronic superficial gastritis tissues were collected and set as gastric cancer group, HGIN group, LGIN group and gastritis group respectively. The expression of CD44v6 and LVD of patients in all the groups were detected using two-step immunohistochemical method to analyze the relationships between the expression of CD44v6 and lymphatic vessel density in early gastric cancer tissues and their relationships with the clinicopathological parameters of patients. The values of LVD in predicting lymph node metastasis in early gastric cancer were evaluated using receiver operating characteristic (ROC) curve.Results:The positive expression of CD44v6 and LVD in the gastritis group, LGIN group, HGIN group and gastric cancer group gradually increased. The positive expression of CD44v6 and LVD in early gastric cancer tissues were in no correlation with the gender, age, tumor site, maximum diameter, differentiation degree and invasion depth (P>0.05) and in a correlation with lymphatic metastasis and lymphatic vessel invasion (P<0.06). The positive expression of CD44v6 in the early gastric cancer tissues was in a positive correlation with LVD (P<0.05). The analysis of ROC curves suggested that the area under ROC curve of predicting lymphatic metastasis of early gastric cancer with LVD was 0.837 (95% CI: 0.756~0.910), and the cut-off value was 14; the corresponding sensitivity and specificity were 63.6% and 90.2 respectively.Conclusion:The expression of CD44v6 and LVD in early gastric cancer tissues are in a close correlation with the clinicopathologic features, and joint detection of expression of CD44v6 and LVD can be taken as the indicator of gastric cancer metastasis.
Highlights
Gastric cancer is one of the common gastrointestinal malignant cancers, and its incidence ranks the second among different malignant cancer in China.[1]
The positive expression of CD44v6 and Lymphatic vessel density (LVD) in the early gastric carcinoma were correlated with lymph node metastasis and lymphatic infiltration (p < 0.05), but in no correlation with gender, age, tumor site, maximum diameter, degree of tissue differentiation and depth of infiltration (P> 0.05, Table-II)
The Spearman rank correlation analysis suggested that the positive expression of CD44v6 in the early gastric cancer tissue was in a positive correlation with LVD (r=0.301, P< 0.05).The receiver operating characteristic (ROC) curve analysis
Summary
Gastric cancer is one of the common gastrointestinal malignant cancers, and its incidence ranks the second among different malignant cancer in China.[1]. It was found that CD44v6 was related to the occurrence and development of malignant cancers such as gastric cancer and colorectal cancer, and its expression could increase at the precancerous stage or early stage of some malignant tumors.[6,7] few studies related to the changes of CD44v6 expression in early gastric cancer tissues are available. The increase of LVD may have a certain predictive value for lymph node metastasis of early gastric cancer. The expressions of CD44v6 and LVD in the early gastric cancer were observed. The relationship between CD44v6 expression, LVD and the clinicopathological parameters of the patients was analyzed to explore the role of them in the occurrence and development of gastric cancer
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