Abstract
BackgroundMany previous studies demonstrated that cell adhesion molecules CD44v6 and integrin-β1 had been extensively investigated as potential prognostic markers of various cancers. However, data in PC are scarce.MethodsWe now investigate CD44v6 and integrin-β1 mRNA expression in PBMC by a triplex real-time RT-PCR assay and protein expression in plasma by ELISA. All specimens were collected from 54 PC patients who received the treatment of cryosurgery as well as 20 healthy individuals (control).ResultsThe mRNA and protein expression levels of CD44v6 and integrin-β1 in patients were significantly increased compared with control group (P<0.05). The high CD44v6 mRNA and protein expression were significantly correlated with clinical stage, tumor differentiation, LNM, liver metastasis and decreased median DFS (P<0.05), while the high integrin-β1 mRNA and protein expression were significantly correlated with clinical stage, LNM, liver metastasis and decreased median DFS (P<0.05). Clinical stage, LNM, liver metastasis, CD44v6 mRNA and protein expression were the independent predictors of survival in PC patients (P<0.05). Moreover, CD44v6 and integrin-β1 mRNA and protein expression levels were significantly decreased in patients in 3 months after cryosurgery (P<0.05). No significant difference was found in CD44v6 mRNA and protein expression between patients in 3 months after cryosurgery and control group (P>0.05).ConclusionCD44v6 and integrin-β1 mRNA and protein expression in blood may serve as biomarkers for the development and metastasis of PC, and as prognostic indicators for PC. They may become useful predictors in assessing outcome of PC patients after cryosurgery.Virtual slidesThe virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4035308681009006.
Highlights
Pancreatic cancer (PC) is one of the most lethal and aggressive human solid malignancies
Since tissue specimens must be removed by tumor resection or biopsy, we often can not prepare enough specimens for the detection of biomarker expression, which is essential for the metastasis and prognosis evaluation of many tumors
The mRNA and protein expression levels of CD44v6 and integrin-β1 in 54 PC patients and 20 healthy individuals were determined by the triplex quantitative real-time RT-PCR and quantitative Enzyme linked immunosorbent assay (ELISA) assay
Summary
Pancreatic cancer (PC) is one of the most lethal and aggressive human solid malignancies. It has been confirmed that argon-helium cryosurgery technology is an effective way to treat benign and malignant tumors, especially the tumors which can not be resected by surgery. This technology has been adopted to treat liver cancer, lung cancer and colorectal cancer [6,7,8,9]. These studies indicated that in 24 colorectal cancer patients performing cryosurgery, the 1- and 3-year survival rates were respectively 90.8% and 59%.
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