Expression of CD44H in the cells of neural crest origin in peripheral nervous system.

Abstract

We investigated the expression of the adhesion molecule CD44 in rat peripheral nervous system (PNS) at the protein and mRNA levels. Most migrating neural crest cells strongly expressed CD44, in contrast to the lack of expression in the neural tube. In dorsal root ganglion (DRG) and sciatic nerve, the distribution of CD44, neurofilament (NF) and S100 suggested the localization of CD44 on the membrane of Schwann cell and neurones and in extracellular matrix (ECM). The expression of CD44 was also confirmed on the membrane of cultured neurones and Schwann cells from DRG. mRNA coding for the haematopoietic form of CD44, CD44H, was detected in neural crest cells, DRG neurones and Schwann cells. These results show that CD44 may play some role in migration of neural crest cells and myelination in terms of adhesion between Schwann cells, axons and ECM.