Expression of CD133 and CD105 on cells in peripheral blood of patients with primary hepatocellular carcinoma

Abstract

Objective To investigate the relationship of CD133+and CD105+cells in the peripheral blood with the clinical prognosis of patients with primary hepatocellular carcinoma(HCC).Methods The proportions of CD45-CD133+CD105+cells were determined in 80 primary HCC patients and 20 healthy subjects by flow cytometry,and the correlation of the proportion with age,gender,clinical stage,histological differentiation,lymph node metastasis,and surgical outcomes were analyzed.The survival rate was analyzed by Kaplan-Meier curves,and multivariate analysis was performed using Cox proportional hazards model.Results The proportion of CD45-CD133+CD105+cells in the peripheral blood of primary HCC patients was significantly higher compared with those in the postoperative patients and healthy controls(P=0.003,P=0.000).The proportion of preoperative CD45-CD133+CD105+cells in the postoperative recurrent group was significantly higher than those in the non-recurrent group(P=0.015).The proportion of CD45-CD133+CD105+cells in the peripheral blood of primary HCC patients was significantly associated with the clinical stage,degrees of differentiation,and presence/absence of lymph node metastasis(P0.05),and not associated with age or gender.CD133 expression was positively correlated with CD105expression(r=0.846,P0.05).Postoperative survival rate was significantly lower in patients with CD45-CD133+CD105+proportion 0.5% compared with≤ 0.5%(P0.05).The postoperative survival rate of patients with CD45-CD133+cell proportion1.1% was significantly lower than that with CD45-CD133+cell proportion ≤1.1%(P0.05).The postoperative survival rate of patients with CD45-CD105+proportion 36% was significantly lower than those with CD45-CD105+proportion ≤ 36%(P0.05).Peripheral blood CD45-CD133+CD105+cell proportion,degree of differentiation,and presence/absence of lymph nodemetastasis were the independent factors influencing HCC prognosis.Conclusion Proportion of CD45-CD133+CD105+cells in the peripheral blood is greatly increased in HCC patients,which is closely associated with the degree of malignancy of HCC.Decreased CD45-CD133+CD105+cell proportion in the peripheral blood is an independent favorable prognostic factor for HCC patients,and targeted lowering of CD45-CD133+CD105+cell proportion in the peripheral blood of primary HCC patients may represent a new treatment.