Expression of carcinoembryonic antigen-related cell adhesion molecule 6 in human hilar cholangiocarcinoma cell line QBC939 and its role in invasion and metastasis

Abstract

Objective To explore the expression of carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) in human hilar cholangiocarcinoma cell line QBC939 and its roles in invasion and metastasis.Methods Real-time polymerase chain reaction (real-time PCR) was used to detect the expression of CEACAM6 in QBC939 cells.Lentiviral-mediated CEACAM6 small interfering RNA (siRNA) was established to infect QBC939 cells.The silencing effect was assayed by real-time PCR.The invasive activity of infected QBC939 cells was analyzed by Transwell invasion assay.Results There was high expression of CEACAM6 in QBC939 cells.Recombinant lentiviral vector expressing siRNA targeting CEACAM6 gene was established successfully and confirmed by PCR and DNA sequencing.The expression of CEACAM6 mRNA in QBC939 cells infected with LV-CEACAM6-siRNA was decreased by 93.1％ as compared with control group (P ＜ 0.05).After CEACAM6 gene was knocked down,Transwell test revealed that the invasion potency of QBC939 cells was significantly suppressed by 69.2％ as compared with control group (P ＜ 0.05).Conclusion There was high expression of CEACAM6 in QBC939 cells.There is a positive correlation between the expression level of CEACAM6 and the invasion potency of hilar cholangiocarcinoma. Key words: RNA interference; Carcinoembryonic antigen-related cell adhesion molecule 6; Hilar cholangiocarcinoma