Abstract
In this study an attempt was made to find out new tissue specific lectin from the seeds of Canavalia gladiata and to investigate whether this could be used as biological markers in haematological malignancies. Lectin binding studies showed that Lymphoid leukemias such as ALL (Acute Lymphocytic Leukemia) and CLL (Chronic Lymphocytic Leukemia) cells did not show any reactivity with CGL (Canavalia gladiata lectin). Blast cells of myeloid leukemias showed varying intensities of staining. Kruskal Wallis One Way ANOVA showed significance in expression of the lectin with different types of Acute Myeloid Leukemia. Lectin cytochemical studies in leukemia revealed its usefulness in differentiating lymphoid leukemias from myeloid leukemias.
Highlights
Leukemia is an unregulated proliferation of haematopoietic tissue that progressively displaces normal blood cell elements
In this study Horseradish peroxidase conjugated Canavalia gladiata lectin was used to evaluate its binding property to different types of leukemic cells and to investigate whether this lectin could be used for the typing of leukemias
One and a half gram of Sephadex G-200 was soaked in distilled water one day before the day of conjugation and on the day it was packed in a long column (75x0.5cm) and washed with Phosphate Buffered Saline (PBS)
Summary
Leukemia is an unregulated proliferation of haematopoietic tissue that progressively displaces normal blood cell elements. Leukemias are traditionally classified by the predominant cell type involved and the degree of maturity or immaturity that characterizes the peripheral blood cell pattern. Plant lectins are of great use in the detection of the complex carbohydrates of the cell membrane. Lectins are valuable cyto and histological tools for the localization of defined carbohydrate determinants. Horse Radish Peroxidase labelled lectins were used for the histochemical distinction of glycolipids with various terminal hexoses in different storage diseases as well as for the demonstration of differences in the composition of cell surface and intercellular glycoproteins in tissues at various stages of differentiation, in malignancy and in functional subset of cells. In this study Horseradish peroxidase conjugated Canavalia gladiata lectin was used to evaluate its binding property to different types of leukemic cells and to investigate whether this lectin could be used for the typing of leukemias
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
