Expression of c- kit, a proto-oncogene of the murine W locus, in cerebella of normal and neurological mutant mice: Immunohistochemical and in situ hybridization analysis

Abstract

Abstract The c- kit proto-oncogene encodes a receptor tyrosine kinase and is allelic with the murine white-spotting ( W) locus. Although no apparent defects in the brain have been reported in W mutant mice, brain tissue, especially cerebellum, shows a high level of c- kit transcription. In the present study, sites of c- kit expression in the cerebellum were exained by immunohistochemical and in situ hybridization techniques. Immunohistochemistry with a monoclonal antibody against c-Kit protein revealed that the c-Kit protein was localized close to the Purkinje cell soma in the region facing the granular cell layer. Similar distribution of the c-Kit protein was observed in cerebella of mutant mice in which the Purkinje cell ( pcd) or the granular cell layer ( weaver) is missing. These data suggest that the c-Kit protein is produced not by the Purkinje cell nor by the granular cell but by the cells present in the molecular layer and that the protein is then transported to the region around the Purkinje cell soma. This interpretation was supported by in situ hybridization analysis: cells containing the c- kit transcripts were found only in the molecular layer, while the granular and Purkinje cells were negative.