Expression of c-Jun N-terminal kinase and B cell lymphoma/leukemia-2 associated X protein in rabbit basilar artery aneurysm

Abstract

Objective To observe the expression of c-Jun N-terminal kinase(JNK) and B cell lymphoma/leukemia-2 associated X protein (bax) during the formation of basilar aneurysm in the rabbit. Methods Forty New Zealand white rabbits were divided into five group, Normal group (n=5), Sham group (n=5) , Operation group(2 weeks, 8 weeks, 16 weeks)(n=10). The bilateral common carotid artery was exposed in the sham operation group by bilateral common carotid artery ligation. MRA (Magnetic Resonance Angiography) was performed at the prescribed time, All experimental animals were killed in order to observe the pathological staining of the basilar artery tip morphology, vascular intima, the middle elastic layer and the immunohistochemical staining. Results The results of immunohistochemical staining showed that the positive rate of JNK in each group was (8.600±2.074)% in the control group, (18.900±4.458)% in the 2W group (t=13.405, P=0.000), and the postoperative 8W group (25.800±3.615)% (t=22.570, P=0.000), postoperative 16W group (38.400±8.343)% (t=14.555, P=0.000); the difference between the experimental group and the control group was statistically significant. The positive expression rates of bax were: (9.400±3.050)% in postoperative 2W group, (22.100±4.748)% in postoperative 2W group (t=14.719, P=0.000) postoperative 8W group (29.200±4.894)% (t=18.866, P=0.000), postoperative 16W group (36.700±4.762)% (t=24.371, P=0.000); each experimental group was statistically significant difference from the control group. Imaging results: aneurysm The volume was: control group (7.600±3.847), (24.746±27.813) mm3,t=2.814, P=0.020), postoperative 8W group (37.083±35.695) mm3 (t=3.285, P=0.009), postoperative 16W group (105.751±97.094) mm3 (t=3.444, P=0.007); the difference between the experimental group and the control group was statistically significant. Conclusion C-Jun N-terminal kinase and bax are involved in the formation of basilar artery aneurysm in rabbits. Key words: Intracranial aneurysm; c-Jun N-terminal kinase; B cell lymphoma/leukemia-2 associated X protein