Expression of C/EBPα and PU.1 Gene In MDS and Their Significance Analysis

Abstract

AbstractAbstract 4649 ObjectiveThis study was aimed to investigate the expression levels and significance of CCAAT/enhancer binding protein alpha gene and transcription factor PU.1 gene in myelodysplasyic syndromes (MDS). MethodsA real time quantitative reverse transcriptase polymerase chain reaction (Real-time PCR) method was used to detect the expression levels of C/EBPα mRNA in bone marrow mononuclear cells (BMMNCs) of 33 patients with MDS and 14 normal controls, Reverse transcription polymerase chain reaction (RT-PCR) was established for detecting PU.1 mRNA expression levels in BMMNCs of above-mentioned samples. ResultsThe expression of C/EBPα was significantly lower in low-risk and high-risk MDS than those of normal controls (P<0.01, P<0.001, respectively), moreover, high-risk MDS showed lower C/EBPα expression than those of low-risk MDS (P<0.05). The expression level of PU.1 significantly decreased in low-risk MDS, as compared with normal controls and high-risk MDS (P<0.001), but there was no significant difference in expression level of PU.1 between high-risk MDS and normal controls (P>0.05). Conclusionreduced C/EBPα and PU.1 expression level have closely associated with the pathogenesis of MDS, they may be important molecular biological markers of MDS; The degree of down-regulated C/EBPα has closely related to the progression of MDS, the detection of C/EBPα gene expression level may be useful for assessing disease progress and prognostic of patients with MDS. Disclosures:Ding:Anhui Provincial Natural Science Foundation (070413257X): Research Funding; Fund of Anhui Provincial “115” Industrial Innovation Program: Research Funding.