Abstract
Bovine prochymosin (bPC) could act to initiate milk clotting. NICE (Nisin Controlled gene Expression system) in lactococcus lactis was one of the most successful and widely using Grampositive gene expression systems. It was composed by six experiments in this study. They are cloning of bovine preprochymosin gene, electrotransformation in lactococcus lactis, construction of expression vector of bPC gene, detection of mRNA after recombinant bPC transcription, identification of recombinant bPC by SDS-PAGE and Western blot. Through the above method, we successfully constructed expression vector of recombinant bPC in lactococcus lactis by using NICE successfully, as well as achieved its expression and identified to immunocompetence of recombinant bPC.
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