Abstract
Objective: To quantified the expression of the morphogenetic bone protein II (BMP II) produced by human cells with osteoblast differentiation, after cultured over dense or porous samples of pure titanium grade II. Material and methods: The samples were produced by powder metallurgy technique. Mesenquimal steam cells isolated from alveolar bone of healthy donors were stimulated to differentiate, assuming an osteoblastic phenotype, by supplemented medium and plated over the samples. The experimental groups were: control, dense titanium, titanium of higher porosity and titanium of lower porosity. After 7 and 14 days, the RNA was extracted. The cDNA was prepared and the polymerase chain reaction (PCR) technique was used in real time, using the constitutive gene Beta-Actin to relative quantification. The pore morphology and interconnection were confirmed by Scanning Electron Microscopy (SEM). Results: Total porosity (obtained after apply dimensions and density formulas) and surface porosity (SEM) presented significant differences among the groups. For the group of total porosity of 33.79%, the superficial porosity was 32.5% (± 7.74%) and for the group of 41.79%, the superficial porosity was 37.4% (± 7.95%), significantly lower. The expression of BMP II was similar in all groups. Conclusion: It was concluded that the powder metallurgy has a reduced ability to standardize the porosity in the samples and that the porosity does not interfere in the cellular response of BMP II production, an important inducer of osteoblastic differentiation. KeywordsPorous titanium; BMP II; Cell culture; PCR.
Highlights
The topography of the titanium surface can increase osteoblast differentiation due to regulation ofgene transcription andexpression of key osteogenic factors in osseointegration
At 7 days of culture, the analysis of variance by ANOVA showed no significant difference between Bone morphogenetic protein (BMP) II expression by the cells in contact with each type of sample, with p = 0,2979.At 14 days, there was no statistical significance in the Bone morphogenetic protein II (BMP II) expression in each group, with p = 0.7669
The distance of 8 percentage points and 5 percentage points did not modify the properties of the two groups of porous titanium samples made by powder metallurgy
Summary
The topography of the titanium surface can increase osteoblast differentiation due to regulation ofgene transcription andexpression of key osteogenic factors in osseointegration. The high porosity facilitates the diffusion of body fluids, favors the migration of cells into the implant and promotes greater amount of bone tissue growth since it increases the area of contact[4], there must be a balance between the rate of porosity and the mechanical properties of the material. Modification of topography influences the expression of molecules by osteoblasts directly affecting their growth and differentiation, and BMPII, which is part of the family of growth factors, has been the subject of research studies that modify titanium. The present study was designed to quantified the expression of the morphogenetic bone protein II (BMP II) produced by human cells with osteoblast differentiation, after cultured over dense or porous samples of pure titanium grade II
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