Abstract
Amelanchier laevis shoots were co-cultured with Agrobacterium tumefaciens carrying the binary vector pBI121, which encodes the B-glucuronidase (GUS) and neomycin phosphotransferase genes. Shoots were then rinsed briefly in liquid MS medium and plated onto culture medium containing carbenicillin. After approximately three months of culture, adventitious shoots were assayed for the presences of GUS by Southern blotting and histochemical assays. Southern analysis revealed a signal when genomic DNA from putatively transformed plants was hybridized with a probe from a segment of pBI121. Regenerated shoots also showed a deep blue color when incubated with X-gluc, although the expression was chimeric, that is, the activity appeared only in a percentage of the cells in each shoot. Callus tissue at the base of each shoot also showed high levels of GUS expression using a fluorogenic assay.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
