Expression of antioxidant oxidoreductases and protein profile of seedling tissues of winter and spring forms of cereals under extreme temperature fluctuations

Abstract

Cultivation of seedlings of soft wheat and barley under extreme temperature fluctuations led to a decrease in the total content of acidic proteins in tissues by more than 25% and reduced the total activity of peroxidases and superoxide dismutases. Against the background of changes in the general expression of enzymes, there was a redistribution of activities between their individual isoforms. Extreme ambient temperatures did not lead to a significant change in the ratio of peroxidase activity to the activity of superoxide dismutase in the cells of seedlings, but it caused a change in the ratio of peroxidase and oxidase activity in individual fractions of the electrophoretic spectrum. The dependence of the expression of structural oxidoreductase genes from the genetically determined type of development (winter or spring) was determined by the “organism-environment” definite situation. When growing cereals at a constant optimum temperature, there was no correlation between the type of development (winter or spring) and quantitative and qualitative indicators of the oxidoreductase spectra. However, when forming a plant stress response to extreme fluctuations in temperature, the degree of expression of some isoforms of enzymes in winter and spring genotypes was different, which indicates the dependence of the functional state of the studied enzymes on the allelic composition of the Vrn1 locus.