Expression of Amylin and Preptin in Iraqi Patients with Type 2 Diabetes Mellitus

Abstract

This study was planned to investigate the relationship between serum amylin and preptin levelsin patients with T2DM compared to healthy controls. And to study their associations together (preptin and amylin) with serum levels of insulin and HOMA-IR. Sixty-four (64) patients were recruited from the Endocrine Outpatient clinic in Al-Imamain Al-Kadhimain city hospital from March of 2018 to May of 2018. Fasting serum samples were obtained on enrolment. (Mean age, (61.73± 8.05) years;mean duration of diabetes, (8.77±2.66) years; mean HbA1c, (8.1 ± 1.7)withT2DM and body mass index (BMI) > 25.1 kg/m2 underwent examination. Age, sex and (BMI) matched with thirty eight(38) healthy controls were also in ruled, Serum preptin and amylin levels were measured by ELISA technique. There was statistically significant difference between patients and controls serum amylin (p=0.023) and preptin (p=0.01).Patients with T2DM had significantly higher blood glucose (p=0.0001), HbA1c (p=0.0001), insulin (p=0.0001), and homeostaticmodel assessment of insulin resistance (HOMA-IR) (p=0.001)compared to healthy control, while total cholesterolwas positively related (p=0.111)triglyceride, low-density lipoprotein cholesterol values were (p<0.001 for each), and significantly lower high-density lipoprotein cholesterol levels comparedwith the control group (p<0.0001). The preptin level demonstrated asignificant positive association with insulin and HOMA-IR compared with healthy control. For healthycontrol group: r=0.381, p=0.146, r=0.133, p=0.438respectively; for T2DM group: (r=0.411, p=0.02, r=0.332, p=0.003), The amylin level also showed a significant positive correlation with insulin and HOMA-IR compared with healthy control. (for healthy control group: r= 0.188, p=0.309, r=-0.039, p=0.911) respectively; for T2DM group: (r=0.279, p=0.002, r=0.291, p=0.003), Conclusions: There were significant differences between healthy control and patients with T2DM concerning amylin and preptin levels. Serum Amylin and preptin increase in association with insulin in diabetic conditions. The present study suggests a potential role of amyline and preptin in the pathogenesis of T2DM.