Abstract
The aim of the study was to identify a set of discriminating genes that could be used for the prediction of Lymph node (LN) metastasis in human colorectal cancer (CRC), and for this, we compared the whole genome profiles of two CRC cell lines (the primary cell line SW480 and its LN metastatic variant, SW620) and identified eight genes [S100 calcium-binding protein P; aldo–keto reductase family 1(AKR1), member B1 (aldose reductase; AKR1B1); AKR1, member C3 (AKR1C3); calponin 3, acidic; metastasis associated in colon cancer 1; hemoglobin, epsilon 1; trefoil factor 3; and FGGY carbohydrate kinase domain containing]. These genes were examined by quantitative RT-PCR in tissues and LNs in 14 CRC patients and 11 control patients. The level of AKR1C3 mRNA expression was significantly different between the Dukes’ stage A, B, and C groups and the control group (p < 0.05, p < 0.001, and p < 0.001) and was also significantly different between Dukes’ stage C and A or B groups (p < 0.05 and p < 0.001, respectively). The expression of CNN3 was significantly different between the Dukes’ stage C and B or control groups (p < 0.001 and p < 0.01, respectively). There were significant correlations between the expression levels of AKR1C3 and CNN3. AKR1C3 and CNN3 expressions are more accurate and suitable markers for the diagnosis of LN metastasis than the other six genes examined in this study.
Highlights
Lymph node (LN) evaluation is an important factor for determining prognosis in colorectal cancer (CRC)
The aim of the study was to identify a set of discriminating genes that could be used for the prediction of Lymph node (LN) metastasis in human colorectal cancer (CRC), and for this, we compared the whole genome profiles of two CRC cell lines and identified eight genes [S100 calcium-binding protein P; aldo–keto reductase family 1(AKR1), member B1; AKR1, member C3 (AKR1C3); calponin 3, acidic; metastasis associated in colon cancer 1; hemoglobin, epsilon 1; trefoil factor 3; and FGGY carbohydrate kinase domain containing]
Our recent study suggests that the expression of the E74-like factor 3 (ELF3) gene in LNs signals the possibility of metastases and that ELF3 may be more suitable than Carcinoembryonic antigen (CEA) as a gene marker for the detection of LN metastases from CRC [7]
Summary
Lymph node (LN) evaluation is an important factor for determining prognosis in colorectal cancer (CRC). Clin Exp Med (2015) 15:333–341 metastases cause recurrence of CRC and are related to prognosis and survival [1]. CEA is generally used for the detection of LN metastases in CRC [3, 4]. Detection of cytokeratin-20 by RT-PCR in peritumoral, histopathologic, tumor-free LNs is an independent prognostic factor for overall survival in CRC [5]. Detection of CXCL10 as a prognostic marker for advanced stage CRC patients may help predict clinical outcomes [6]. Our recent study suggests that the expression of the E74-like factor 3 (ELF3) gene in LNs signals the possibility of metastases and that ELF3 may be more suitable than CEA as a gene marker for the detection of LN metastases from CRC [7]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
