Abstract
背景与目的肿瘤的复发和耐药是肿瘤患者死亡的主要原因。乙醛脱氢酶（acetaldehyde dehydrogenase, ALDH）家族与肿瘤细胞的增殖、迁移、侵袭和耐药密切相关，且ALDH不同亚型基因在不同肿瘤细胞中有差异性表达。本实验旨在分析对吉非替尼耐药的人肺腺癌细胞HCC-827/GR ALDH亚型的表达。方法利用人肺腺癌细胞系HCC-827制备吉非替尼耐药细胞株HCC-827/GR；利用流式检测HCC-827及HCC-827/GR中ALDH的表达；采用MTT法检测ALDH抑制剂二乙氨基苯甲醛（diethyllaminaldehyde, DEAB）处理前后HCC-827/GR细胞的增殖能力和对吉非替尼的敏感性；利用qRT-PCR检测HCC-827与HCC-827/GR细胞中ALDH各亚型在mRNA水平的表达。结果与HCC-827细胞相比，ALDH在吉非替尼耐药的细胞株HCC-827/GR的阳性率增加；经100 μmol/L DEAB处理后，HCC-827/GR细胞增殖能力下降；与HCC-827细胞相比，ALDH1A1和ALDH1L1在HCC-827/GR细胞中mRNA表达水平增高；ALDH3B2表达降低。结论ALDH具有检测吉非替尼耐药的人肺腺癌细胞的标志分子的潜力，其中ALDH1A1可能参与人肺腺癌细胞对吉非替尼耐药性的形成过程。
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