Abstract
An antigen immunologically cross-reacting with the murine mammary virus major glycoprotein (gp52) was found on the cell surfaces of a human breast cancer cell line (T-47D) and its subcloned derivatives. The presence of this antigen was established by various immunological methods: indirect immunoperoxidase technique on live and fixed cells, indirect radioimmunoassay of live cells and immunoscanning electron microscopy. We therefore propose the T-47D cell line and its subclones as an in vitro model system for studying the modulation of expression of this tumor-associated antigen.
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