Abstract
ABSTRACT An upstream 4.8 kb region of the soybean (Glycine max [L.] Merr.) seed lipoxygenase-3 gene was linked to the coding sequence of the bacterial reporter gene β-glucuronidase and expression of the chimeric construct was monitored in transgenic tobacco plants. Quantitative fluorometric analysis showed the gene to be expressed most highly in seed, followed by moderate to low expression in non-seed tissues, such as hypocotyl and cotyledon, stem, leaf, and root. Histochemical localization revealed expression in rapidly dividing cells of transgenic seedlings, such as root tip and cotyledon. In mature vegetative plants, however, expression of Lox3:GUS was limited principally to epidermal layers, including epidermal hairs. Among the reproductive structures, Lox3:GUS expression was observed in pollen and in the stigmatic surface.
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